檢測認證人脈交流通訊錄
丙型肝炎病毒NS3（解旋酶）蛋白片段
Catalog Number: HCV-NS3h
產品目錄號：HCV-NS3h
Genotype: 2a
基因型：2a
Form: Purified via Nickel affinity chromatography
形式：鎳柱親和層析純化
Tag: Expressed with a C-terminal 6X His-tag
標簽：C端6X His-tag表達
Origin: E.coli
來源：E.coli
Applications: ELISA、Flow Cytometry、SDS-PAGE、Western blot
應用：酶聯免疫吸附實驗（ELISA）、流式細胞分析（Flow Cytometry）、SDS-PAGE、免疫印跡（Western blot）
Buffer/Composition: 1X PBS pH 7.2; 1X protease inhibitor cocktail, 1 mM PMSF
緩沖液/成分：1X PBS pH 7.2；1X protease inhibitor cocktail；1 mM PMSF
Volume: 50μl/ 250μl/ 500μl
體積：50μl/ 250μl/ 500μl
Concentration: 2 μg/μl
濃度：2 μg/μl
Storage: Short Term: 4℃ Long Term (up to 1 year): -20℃(avoid repeated freeze/thaw)
儲存：短期：4℃ 長期（可達1年）：-20℃（避免反復凍融）
Coomassie stained gel showing purification of recombinant NS3 helicase protein.Bacterial cells expressing the His-tagged protein were lysed (lane 2), pelleted, and the SN (lane 3) was loaded onto Nickel resin. The ~55 kD protein was absent in the resin flow thru (lane 4) as well as column washes (lanes 5 and 6). Elutions with immidazole (lanes 7 and 8) yielded ≥95% pure protein.Lane 1 = marker
考染顯示純化的重組NH3解旋酶蛋白。表達His標簽蛋白的菌細胞裂解物（lane 2），顆粒狀的SN（lane 3）過鎳樹脂。~55 kD蛋白在樹脂流出液（(lane 4）中消失，同見于柱洗滌液（lanes 5 & 6）。洗脫液連同咪唑（lanes 7 & 8）產生≥95%純度的蛋白質。Lane 1 = marker
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Item #: HCV-NS3h & HCV-NS3h-500 & HCV-NS3h-1
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