THE MONOTRACE ADVANTAGE（優勢）

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of allergen protein contamination in a variety of food samples. MonoTrace ELISAs incorporate the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

1、Target a major allergen resistant to food processing effects

2、Recognize a specific target, thereby eliminating cross-reactivity and reducing the possibility?

? of‘false positive’results

3、Perform well in a wide variety of food products with negligible food matrix interference

?

BioFront Technologies開發了第一條完全以單克隆抗體為基礎的檢測試劑盒產品（MonoTrace ELISAs），檢測各種食物基質中的致敏蛋白污染。MonoTrace ELISAs綜合使用一系列精準確定的經過嚴格篩選過程后仔細挑選的單克隆抗體來實現：

1、分析耐食物加工的主要過敏原

2、特異性識別目標成分，從而消除交叉反應，以及減少“假陽性”結果的可能性

3、在各種食品（食品基質干擾可以忽略不計）的檢測中表現優異

BRAZIL NUT ASSAY PERFORMANCE 巴西堅果試劑盒性能

Overall Assay Time: Now just 30 minutes post-extraction!

Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.

Number of Tests per Kit: 96

*Limit of Detection: 0.2 ppm

*Range of Quantification: 1 to 40 ppm

Specificity: Brazil Nut

*= calculations of parameters are based on representative data from ten assays, however, users should consult accompanying certificate of analysis for lot-specific values.

實驗總時間：現在抽提后僅需30分鐘！

孵育時間：抽提，樣品孵育，二抗孵育，底物孵育每步僅需10分鐘。

每一試劑盒測試數量：96

*檢測限：0.2 ppm

*定量范圍：1至40 ppm

特異性：巴西堅果

*=參數的計算根據10次代表性的數據得出，然而，使用者應當查閱附隨的分析證書中的具體批次數值。

CROSS-REACTIVITY 交叉反應

At concentrations as high as 10,000 ppm, cross-reactivity (below 1%) was observed in walnut, hazelnut, and pecan.? No cross-reactivity seen with peanut, pistachio, cashew, macadamia nut, and pine nut.

在濃度高達10,000 ppm時，在核桃、榛子和胡桃中觀察到交叉反應（低于1%）。在花生、開心果、腰果、澳洲堅果和松子中無交叉反應。

RECOVERY 回收

Recovery of spiked almond samples at various concentrations was efficient (>90%) when the milk chocolate and dark chocolate were assayed

當分析以下食物基質時，不同濃度巴西堅果樣品的加標回收率很高（>90%）：牛奶巧克力，黑巧克力。