THE MONOTRACE ADVANTAGE（優(yōu)勢）

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of allergen protein contamination in a variety of food samples. MonoTrace ELISAs incorporate the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

1、Target a major allergen resistant to food processing effects

2、Recognize a specific target, thereby eliminating cross-reactivity and reducing the possibility?

? of‘false positive’results

3、Perform well in a wide variety of food products with negligible food matrix interference

?

BioFront Technologies開發(fā)了第一條完全以單克隆抗體為基礎(chǔ)的檢測試劑盒產(chǎn)品（MonoTrace ELISAs），檢測各種食物基質(zhì)中的致敏蛋白污染。MonoTrace ELISAs綜合使用一系列精準(zhǔn)確定的經(jīng)過嚴(yán)格篩選過程后仔細(xì)挑選的單克隆抗體來實(shí)現(xiàn)：

1、分析耐食物加工的主要過敏原

2、特異性識(shí)別目標(biāo)成分，從而消除交叉反應(yīng)，以及減少“假陽性”結(jié)果的可能性

3、在各種食品（食品基質(zhì)干擾可以忽略不計(jì)）的檢測中表現(xiàn)優(yōu)異

BRAZIL NUT ASSAY PERFORMANCE 巴西堅(jiān)果試劑盒性能

Overall Assay Time: Now just 30 minutes post-extraction!

Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.

Number of Tests per Kit: 96

*Limit of Detection: 0.2 ppm

*Range of Quantification: 1 to 40 ppm

Specificity: Brazil Nut

*= calculations of parameters are based on representative data from ten assays, however, users should consult accompanying certificate of analysis for lot-specific values.

實(shí)驗(yàn)總時(shí)間：現(xiàn)在抽提后僅需30分鐘！

孵育時(shí)間：抽提，樣品孵育，二抗孵育，底物孵育每步僅需10分鐘。

每一試劑盒測試數(shù)量：96

*檢測限：0.2 ppm

*定量范圍：1至40 ppm

特異性：巴西堅(jiān)果

*=參數(shù)的計(jì)算根據(jù)10次代表性的數(shù)據(jù)得出，然而，使用者應(yīng)當(dāng)查閱附隨的分析證書中的具體批次數(shù)值。

CROSS-REACTIVITY 交叉反應(yīng)

At concentrations as high as 10,000 ppm, cross-reactivity (below 1%) was observed in walnut, hazelnut, and pecan.? No cross-reactivity seen with peanut, pistachio, cashew, macadamia nut, and pine nut.

在濃度高達(dá)10,000 ppm時(shí)，在核桃、榛子和胡桃中觀察到交叉反應(yīng)（低于1%）。在花生、開心果、腰果、澳洲堅(jiān)果和松子中無交叉反應(yīng)。

RECOVERY 回收

Recovery of spiked almond samples at various concentrations was efficient (>90%) when the milk chocolate and dark chocolate were assayed

當(dāng)分析以下食物基質(zhì)時(shí)，不同濃度巴西堅(jiān)果樣品的加標(biāo)回收率很高（>90%）：牛奶巧克力，黑巧克力。