THE MONOTRACE ADVANTAGE（優(yōu)勢）

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of allergen protein contamination in a variety of food samples. MonoTrace ELISAs incorporate the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

1、Target a major allergen resistant to food processing effects

2、Recognize a specific target,thereby eliminating cross-reactivity and reducing the possibility of

? of‘false positive’results

3、Perform well in a wide variety of food products with negligible food matrix interference

BioFront Technologies開發(fā)了第一條完全以單克隆抗體為基礎(chǔ)的檢測試劑盒產(chǎn)品（MonoTrace ELISAs），檢測各種食物基質(zhì)中的致敏蛋白污染。MonoTrace ELISAs綜合使用一系列精準確定的經(jīng)過嚴格篩選過程后仔細挑選的單克隆抗體來實現(xiàn)：

1、分析耐食物加工的主要過敏原

2、特異性識別目標成分，從而消除交叉反應(yīng)，以及減少“假陽性”結(jié)果的可能性

3、在各種食品（食品基質(zhì)干擾可以忽略不計）的檢測中表現(xiàn)優(yōu)異

MACADAMIA NUT ASSAY PERFORMANCE

夏威夷果分析試劑盒性能

Overall Assay Time: Now just 30 minutes post-extraction!

Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.

Number of Tests per Kit: 48 & 96

Limit of Detection: ＜ 1ppm

Range of Quantification: 2 to 80 ppm

Specificity: Macadamia nut

*= calculations of parameters are based on representative data from ten assays, however, users should consult accompanying certificate of analysis for lot-specific values.

實驗總時間：現(xiàn)在抽提后僅需30分鐘！

孵育時間：抽提，樣品孵育，二抗孵育，底物孵育每步僅需10分鐘。

每一試劑盒測試數(shù)量：48 &?96

檢測限：＜ 1ppm

定量范圍：2至80 ppm

特異性：夏威夷果

*=參數(shù)的計算根據(jù)10次代表性的數(shù)據(jù)得出，然而，使用者應(yīng)當查閱附隨的分析證書中的具體批次數(shù)值。

CROSS-REACTIVITY 交叉反應(yīng)

At concentrations as high as 100,000 ppm, no cross-reactivity was observed in a large panel of assayed samples, including: almond, walnut, hazelnut, pecan, peanut, pistachio, cashew, pine nut, chestnut，coconut and Brazil nut.

在濃度高達100,000 ppm時，對一份大型分析樣品中沒有觀察到交叉反應(yīng)，包括：杏仁、核桃、榛子、胡桃、花生、開心果、腰果、松子、栗子、椰子和巴西堅果。

RECOVERY 回收

Recovery of spiked macadamia nut samples at various concentrations was efficient (>90%) when the following food matrices were assayed: chocolate, cereals, ice cream.

當分析以下食物基質(zhì)時，不同濃度夏威夷果樣品的加標回收率很高（>90%）：巧克力，谷類食品，冰激凌。