檢測認證人脈交流通訊錄
- PHYB / Anti-Phytochrome B antibody
Product Information
Description:
Mouse monoclonal (Clone: 10P5) antibody
Background:
PHYB is a red/far-red photoreceptor involved in the regulation of de-etiolation. It exists in two inter-convertible forms: Pr and Pfr (active). It is involved in the light-promotion of seed germination and in the shade avoidance response.
Synonyms:
PHYB, HY3, OOP1, OUT OF PHASE 1, PHYTOCHROME B
Immunogen:
Recombinant protein of PHYB derived from Arabidopsis thaliana AT2G18790.
Form:
Lyophilized
Quantity:
150 μg
Purification:
Protein A purified
Reconstitution:
Reconstitution with 150μl of 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability &
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Application Information
Applications:
Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.
Expected Results:
125 kDa
Confirmed Reactivity:
Arabidopsis thaliana
Predicted Reactivity:
For more species homologues information, please contact tech support at tech@phytoab.com.
Application Example
Lane 1: 35 μg total protein from phyB-9 mutant of Arabidopsis thaliana leaf.
Lane 2: 35 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 8% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP).
Detection: using chemiluminescence substrate and image were captured with CCD camera.
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