檢測認證人脈交流通訊錄
- HY5 / Anti-Protein long hypocotyl 5 antibody
Product Information
Description:
Mouse monoclonal antibody
Background:
HY5 is a basic leucine zipper (bZIP) transcription factor with nuclear localization. It is involved in light-regulated transcriptional activation of G-box-containing promoters. HY5 plays a role in anthocyanin accumulation in far-red light and blue light, but not in red light or in the dark.
Synonyms:
HY5, ELONGATED HYPOCOTYL 5, REVERSAL OF THE DET PHENOTYPE 5, TED 5
Immunogen:
Recombinant protein of HY5 derived from Arabidopsis thaliana AT5G11260.
Form:
Lyophilized
Quantity:
150 μg
Purification:
Protein A purified
Reconstitution:
Reconstitution with 150μl of 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability &
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Application Information
Applications:
Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.
Expected Results:
18 kDa
Confirmed Reactivity:
Arabidopsis thaliana
Predicted Reactivity:
For more species homologues information, please contact tech support at tech@phytoab.com.
Application Example
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP) (Cat# PHY6006)
Detection: using chemiluminescence substrate and image were captured with CCD camera.
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