檢測認證人脈交流通訊錄
- 正常人主要細胞溶解產物
Preparation:Cell lysate was prepared in modified RIPA buffer (50mMTris, pH7.2, 150mM NaCl, 1mM EDTA, 1mM EGTA, 1% NP-40 , 1mM Na3VO4, 5mM NaF, 400uM PMSF, 1ug/ml of pepstatin A, 5ug/ml of aprotinin, 5ug/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was diluted and boiled for 5 min with nonreducing sample buffer (50 mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue) containing 5% beta-mercaptoethanol.
Concentration:2 mg/ml
Storage: Stable for 6 months at -20oC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
Application: Cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
Cat.No. Product Name Size Price
1106 Human brain vascular smooth muscle cell lysate 100 µg $330
人腦血管平滑肌細胞
1206 Human brain vascular pericyte lysate 100 µg $330
人腦血管周邊細胞
1406 Human meningeal cell lysate 100 µg $330
人腦膜細胞
1526 Human neuron lysate 100 µg $330
人神經元細胞
1606 Human oligodendrocyte precursor cell lysate 100 µg $330
人少突先驅膠質細胞
1716 Human perineurial cell lysate 100 µg $330
人周神經細胞
1806 Human astrocyte lysate 100 µg $330
人星形膠質細胞
1906 Human microglia lysate 100 µg $330
人小膠質細胞
2006 Human dermal microvascular endothelial cell lysate 100 µg $330
人皮膚微血管內皮細胞
2016 Human dermal vascular smooth muscle cell lysate 100 µg $330
人皮膚血管平滑肌細胞
2106 Human dermal kerotinocyte lysate 100 µg $330
人皮膚角質細胞
2206 Human dermal melanocyte lysate 100 µg $330
人皮膚黑素細胞
2306 Human dermal fibroblast lysate 100 µg $330
人皮膚纖維原細胞
2506 Human lymphatic endothelial cell lysate 100 µg $330
人淋巴內皮細胞
2536 Human lymphatic fibroblast lysate 100 µg $330
人淋巴纖維原細胞
2626 Human gingival fibroblast lysate 100 µg $330
人齒齦纖維原細胞
2636 Human peridontal ligmant firbroblast lysate 100 µg $330
人齒根膜韌帶纖維原細胞
2716 Human esophageal smooth muscle cell lysate 100µg $330
人食管平滑肌細胞
2906 Human intestinal microvascular endothelial cell lysate 100 µg $330
人腸微血管內皮細胞
2916 Human intestinal smooth muscle cell lysate 100 µg $330
人腸平滑肌細胞
3006 Human pulmonary microvascular endothelial cell lysate 100 µg $330
人肺微血管內皮細胞
3106 Human pumonary artery endothelial cell lysate 100 µg $330
人肺動脈內皮細胞
3116 Human pulmonary artery smooth muscle cell lysate 100 µg $330
人肺動脈平滑肌細胞
3126 Human pulmonary artery fibroblast lysate 100 µg $330
人肺動脈纖維原細胞
3206 Human pulmonary alveolar epithelial cell lysate 100 µg $330
人肺齒槽上皮細胞
3306 Human pulmonary fibroblast lysate 100 µg $330
人肺纖維原細胞
3406 Human bronchial smooth muscle cell lysate 100 µg $330
人支氣管平滑肌細胞
3416 Human tracheal smooth muscle cell lysate 100 µg $330
人氣管平滑肌細胞
3506 Human skeletal muscle cell lysate 100 µg $330
人骨骼肌細胞
4006 Human renal glomerular endothelial cell lysate 100 µg $330
人腎小球內皮細胞
4106 Human renal proximal tubular epithelial cell lysate 100 µg $330
人腎最接近管狀上皮細胞
4116 Human renal cortical epithelial cell lysate 100 µg $330
人腎皮層上皮細胞
4126 Human renal epithelial cell lysate 100 µg $330
人腎上皮細胞
4206 Human renal mesangial cell lysate 100 µg $330
人腎系膜細胞
4306 Human bladder microvascular endothelial cell lysate 100 µg $330
人膀胱微血管內皮細胞
4316 Human bladder smooth muscle cell lysate 100 µg $330
人膀胱平滑肌細胞
4336 Human bladder stromal fibroblast lysate 100 µg $330
人膀胱基質纖維原細胞
4606 Human calvarial osteoblast lysate 100 µg $330
人顱蓋造骨細胞
5006 Human hepatic sinusoidal endothelial cell lysate 100 µg $330
人肝竇狀腺內皮細胞
5106 Human intraheptic biliary epithelial cell lysate 100 µg $330
人肝膽上皮細胞
5206 Human hepatocyte lysate 100 µg $330
人肝細胞
6006 Human cardiac microvascular endothelial cell lysate 100 µg $330
人心臟微血管內皮細胞
6106 Human aortic endothelial cell lysate 100 µg $330
人大動脈內皮細胞
6116 Human aortic smooth muscle cell lysate 100 µg $330
人大動脈平滑肌細胞
6206 Human cardiac myocyte lysate 100 µg $330
人心肌細胞
6526 Human keratocyte lysate 100 µg $330
人角膜細胞
6546 Human retinal pigment epithelial cell lysate 100 µg $330
人視網膜色素上皮細胞
6556 Human lens epithelial cell lysate 100 µg $330
人視鏡上皮細胞
6566 Human iris pigment epithelial cell lysate 100 µg $330
人虹膜色素上皮細胞
6586 Human non-pigment ciliary epithelial cell lysate 100 µg $330
人非色素睫毛上皮細胞
7106 Human amniotic epithelial cell lysate 100 µg $330
人羊膜上皮細胞
7126 Human villous trophoblast lysate 100 µg $330
人長茸毛滋養層
7136 Human villous mesenchymal fibroblast lysate 100 µg $330
人長茸毛間葉纖維原細胞
7216 Human preadipocyte lysate 100 µg $330
人前脂肪細胞
8006 Human umbilical vein endothelial cell lysate 100 µg $330
人臍靜脈內皮細胞
8016 Human umbilical artery endothelial cell lysate 100 µg $330
人臍動脈內皮細胞
8026 Human umbilical vein smooth muscle cell lysate 100 µg $330
人臍靜脈平滑肌細胞
8036 Human umbilical artery smooth muscle cell lysate 100 µg $330
人臍動脈平滑肌細胞
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